Composite

Part:BBa_K3096031

Designed by: Patrick Mueller, Famke Baeuerle, Jakob Keck, Lina Widerspick   Group: iGEM19_Tuebingen   (2019-10-14)

A constitutively active promoter expresses Clts, a temperature sensitive cI repressor of the lambda phage. If the temperature is significantly below 37°C (body temperature), Clts is an active repressor for the cI lambda promoter. This promoter controls the transcription of a repressor protein, AraC. Therefore, if conditions are not 37°C, the AraC is not available, hence the pBAD promoter, controlled by AraC, is active and genes under the control of the pBAD promoter are not transcribed.

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